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KMID : 0043320090320111565
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Archives of Pharmacal Research
2009 Volume.32 No. 11 p.1565 ~ p.1572
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Functional Activation of Macrophages, Monocytes and Splenic Lymphocytes by Polysaccharide Fraction from Tricholoma matsutake
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Cho Jae-Youl
Byeon Se-Eun
Lee Jae-Hwi
Lee Eun-Ji
Lee Song-Yi
Hong Eock-Kee
Kim Young-Eon
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Abstract
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Mushroom-derived polysaccharides (β-glucans) are considered as a valuable biopharmaceutical principle without displaying side effects. Although Tricholoma matsutake is well-known mushroom in Korea, Japan and China, the immunoregulatory roles of T. matsutake-derived polysaccharides were not fully elucidated yet. In this study, we continued to evaluate the immunomodulatory effect of T. matsutake-derived polysaccharide fraction (TmC-2) using functional activation models of macrophages, monocytes and splenic lymphocytes. TmC-2 treatment strongly increased the production of NO and TNF-α. Phagocytic uptake and ROS generation was also up-regulated by TmC-2. Interestingly, TmC-2 stimulated CD29-mediated cell-cell or cell-finbronectin adhesions in monocytes, while CD43-mediated cell adhesion was down-regulated. Interestingly, the enhancement of proliferation and IFN-γ production was striking observed in TmC-2-treated splenic lymphocytes. The activation seemed to be mediated by up-regulating intracellular signaling cascades such as PI3K/Akt and MAPK (ERK and p38) and by the involvement of surface receptors (dectin-1 and TLR-2). Therefore, our results suggest that this TmC-2 from T. matsutake can be developed as a promising immunostimulatory principle, applicable to people with lowered immunomodulatory potentials.
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KEYWORD
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Tricholoma matsutake, Polysaccharide, Immune response, Macrophage, Monocyte, Splenic lymphocyte
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